18-year-olds Ryan Steele and J.B. Reese are co-employees of the Tao Dojo in Cross World City, as well as best friends with a news reporter named Kaitlin Starr from the Underground Voice Daily. One day, Ryan Steele receives a startling message regarding his missing father, Tyler, from a man named Professor Horatio Hart.

The threesome are called to Professor Hart's laboratory on the outskirts of the city, where they learn that an evil mutant named Grimlord is responsible for the abduction of Ryan's father. Thanks to a recorded message left by Tyler, he and Professor Hart inform Ryan, Kaitlin, and J.B. of a technological marvel that they believe would be able to fight Grimlord and keep the real world safe from his tyranny—the perfect melding of man and machine, which they have named V.R. Troopers.

With special pendants called Virtualizers worn around their necks, Ryan, Kaitlin, and J.B. must transform into action and travel between their world and the world of Virtual Reality to defend the universe from Grimlord and his mutant army.