De Mol is a reality TV show in which ten candidates have to complete assignments as a means to earn money, all of which will go to the winner of the show. However, one person of the group is "De Mol" (The Mole). His goal is to make assignments fail and keep the amount of money earned as low as possible. The nine other candidates have to try and figure out who De Mol is.

At the end of each episode, all contestants have to fill in a quiz about De Mol's identity. The contestant with the lowest number of correct answers is eliminated and removed from the game. This continues until the final test when only two candidates are left next to De Mol. The contestant with most correct answers in the final test can take home all the money earned during the previous episodes.