The Lowe Files follows Rob Lowe and his two sons, Matthew and John Owen, as they travel through the country to explore infamous unsolved mysteries, a curiosity that Rob has had since his early childhood days and has now been passed down to his boys.

In each episode of "The Lowe Files", Rob, Matthew and John Owen seek out a mysterious story or spooky legend and immerse themselves in the exploration and debate of the experience. While fun in spirit, the Lowe's are able to conduct deliberate and scientific investigations on a highly-sophisticated level by meeting with top experts, using high-tech monitoring equipment and taking in-depth training courses.